Follow us...

 

Search News Archives

News Channels

 

New Laboratory Products

Lab News

What's going on at Lab Innovations

Research & Case Studies

Microscopy | Image Analysis

Separation Science

Brochures & Literature

Videos

Events | Webinars

 

 

Conferences | Events

Utilizing Fast HPLC Screening Methods for the Detection of Steroids

publication date: Feb 9, 2015
 | 
author/source: Agilent

As the importance of steroids in the regulation of bioprocesses has increased so has the demand for fast screening methods for their detection.

Agilent technologiesHPLC is a highly regarded analytical technique for pharmaceutical applications due to its unrivalled analytical validation characteristics; including accuracy, precision, limit of detection, specificity, linearity and range, and ruggedness.

The high throughput demands of quantitative steroid analysis require faster run times and as a result there has been a focus on the development of fast HPLC screening methods to detect steroids such as hydrocortisone, testosterone and progesterone. Recent developments in HPLC column technology, such as sub-2μm totally porous and sub-3 μm superficially porous columns, have offered improvements in separation speed and performance through reduced analysis times and increased separation efficiency.  It is these improvements in column technology which mean that fast HPLC screening for the detection of steroids can be achieved.

Selectivity is the most powerful tool to optimize separations in HPLC and can be manipulated to improve separations.   As a result, the analysis of steroids can be dramatically improved by choosing the optimal HPLC column for the separation.  Selectivity can be increased by changing the mobile phase composition, column temperature and the composition of stationary phase. For example, the short column length and high efficiency of Poroshell 120 HPLC columns  provide fast HPLC analysis times and rapid equilibration leading to fast investigations of selectivity.

A key feature of Poroshell 120 columns is their superficially porous  microparticulate column packing.   Poroshell 120 particles have a 1.7 μm solid silica core with a 0.5 μm porous outer layer. This unique configuration delivers all the performance advantages of sub-2 μm particles with the backpressure of a sub-3 μm particle. With Poroshell 120 columns, it is possible to achieve 80-90% or more of the efficiency expected from a sub-2 μm Fast LC/UHPLC column – but at HPLC pressures (below 400 bar). This ability to perform fast separations at low pressures can dramatically enhance productivity by allowing more samples to be run in less time – even using existing HPLC systems. This allows laboratories to generate better chromatographic results quicker so they can increase their throughput of samples. The Agilent LC Column Selection Tool is designed to help you find out the optimal column to help you achieve fast HPLC screening.


more about agilent technologies


more news from agilent technologies


 


If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners