Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

Untangling the Web of Gene Expression

publication date: Jun 11, 2015
 | 
author/source: Integrated DNA Technologies

 Untangling the Web of Gene Expression

IDT facilitates a unique, scalable method for analyzing hundreds of enhancer:promoter interactions in a single assay

Advancing the development of novel methods for understanding gene expression, Integrated DNA Technologies (IDT) has enabled Dr Jim Hughes, Associate Professor at the Weatherall Institute of Molecular Medicine, Oxford, UK, to optimize his unique Capture-C method, based on Chromosomal Conformational Capture (3C). As outlined in the recent DECODED Online article, Understanding How Distal Regulatory Elements Control Gene Expression,  Dr Hughes uses IDT XGen® Lockdown® Probes to generate highly adaptable target capture probe pools for determining enhancer:promoter interactions, allowing greater flexibility in experimental design.

With enhancers and other regulatory elements often lying 10s to 100s of kilobases from the genes they affect, understanding the regulation of gene expression is an incredibly complex endeavor. The article describes how Dr Hughes and his collaborators have developed a versatile and scalable solution to enrich for enhancer:promoter interactions by integrating oligo capture and NGS technology into the 3C workflow. Off-the-shelf probe pools or panels often do not match a researcher’s experimental needs, while custom designed probe pools or panels often come at a high cost. Instead, Dr Hughes has utilized IDT’s xGen Lockdown Probes, scaled to create affordable custom probe pools to target specific regions of interest in the genome. He is able to modify the target and perform multiplex assays easily by mixing and augmenting pools with additional probes to create new panels, as required. Using this method, hundreds of genes can be interrogated in the same assay.

Developing this new method has allowed Dr Hughes and his team to enhance their workflow while saving costs as their research interests evolve. Their work has already brought new insight into the currently accepted “looping model” of promoter:enhancer interaction, and has secured Capture-C as a prominent method for addressing gene regulation.

Read the DECODED article to find out more about how Dr Hughes has used xGen Lockdown Probes to greatly improve his Capture-C protocol. For more information please visit www.idtdna.com/xgen. Follow us on twitter @idtdna for real-time updates and insights.


more about integrated DNA technologies


more news from integrated DNA technologies


 


If you have not logged into the website then please enter your details below.



 

News Channels

 

 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 

 

 

 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events