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Tools for Post Purification Sample Handling

publication date: Oct 16, 2015
 | 
author/source: Genevac Ltd

Genevac centrifugal evaporator

Genevac, specialists in solvent removal, have developed a suite of proprietary technological solutions to facilitate post purification sample handling, including reformatting.

For labs undertaking purification using Normal Phase HPLC, SFC or Flash chromatography – removal of organic solvents can be simply, productively and safely carried out in a Genevac centrifugal evaporator (EZ-2, ROCKET, Rocket Synergy or HT Series III).  Where the nature of the sample being concentrated can lead to solvent bumping (e.g. natural products) - Genevac's DriPure® technology is proven to completely eliminate this troublesome and time-consuming problem

The evaporation of aqueous acetonitrile (or methanol / ethanol) fractions resulting from 

Reverse Phase HPLC is a widely carried out application on many Genevac evaporator systems.  A multiple stage method has been developed for the EZ-2 evaporator to remove the organic solvent without freezing the water, remove the water, and then dry any remaining stubborn solvent.  Alternatively, the unique LyoSpeed™ fast lyophilisation method can be used in Genevac HT series evaporators to produce dry (solvent-free) powders from samples that previously could only be produced as gums and oils.  Recent advances in condenser technology, in particular the high power -75°C cold trap, available on HT Series III and Rocket Synergy evaporators, has led to significant advances in lyophilisation success rates.

During post purification handling - samples typically need to be reformatted from fraction tubes or flasks, into a small vial for compound storage. For this specific time consuming task, Genevac developed SampleGenie™ technology.  SampleGenie™ enables a large volume to be dried (or lyophilised) directly into a small storage vial in a fraction of the time taken by traditional evaporation methods that require you to reformat samples from a flask or multiple tubes into a vial.

Genevac, now part of SP Scientific, was founded in 1990.  Today the company employs around 65 people, with manufacturing, R&D and marketing headquartered in Ipswich, UK. Genevac offers a comprehensive portfolio of evaporators to suit almost any solvent removal application, purchasing budget or productivity requirement.


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