Follow us...

 

Search News Archives

Monthly Newsletter

 

Lab Bulletin May Newsletter

view the latest issue

Subscribe

News Channels

 

New Laboratory Products

 

Lab News

 

Microscopy & Image Analysis

 

Separation Science

 

Research & Case Studies

 

Literature

 

Videos

 

Events | Webinars

 

 

 

Conferences | Events

Reducing PCR reaction times by using faster ramp rates, modifying programs and changing the reaction mix

publication date: May 17, 2010
 | 
author/source: Bibby Scientific Limited

In the modern laboratory, speed is often of the essence and any process capable of saving valuable research time is an advantage. One recent advance in this direction is the development of fast PCR protocols which include both specialised PCR reagents and modified thermal cycling steps. The Techne TC-PLUS thermal cycler gives the user all the features required to make best use of these new methodologies by offering flexible programming and rapid block ramp rates of up to 5ºC/s.

The factors that determine the length of a PCR run are the number of steps and cycles, including any initial denaturation/enzyme activation and final extension steps, the hold times of these steps and the ramp rates between them. All these factors need to be considered when optimising for fast PCR protocols. This application note demonstrates some of the time savings that can be made by changing some of these parameters.

To read the full article please click here


If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners