Search News Archives
Follow us on
Conferences | Events

Media Partners


Reducing PCR reaction times by using faster ramp rates, modifying programs and changing the reaction mix

publication date: May 17, 2010
author/source: Bibby Scientific Limited

In the modern laboratory, speed is often of the essence and any process capable of saving valuable research time is an advantage. One recent advance in this direction is the development of fast PCR protocols which include both specialised PCR reagents and modified thermal cycling steps. The Techne TC-PLUS thermal cycler gives the user all the features required to make best use of these new methodologies by offering flexible programming and rapid block ramp rates of up to 5ºC/s.

The factors that determine the length of a PCR run are the number of steps and cycles, including any initial denaturation/enzyme activation and final extension steps, the hold times of these steps and the ramp rates between them. All these factors need to be considered when optimising for fast PCR protocols. This application note demonstrates some of the time savings that can be made by changing some of these parameters.

To read the full article please click here

If you have not logged into the website then please enter your details below.


Popular this Month


Win a PIPETBOY from the new Integra summer collection!


Great Savings off Selected GC Columns


CytoSMART raises 5.2 million Euros for further product innovation and growth


High Volume Sample Storage in Space Efficient 96-Well Format


Cherwell Publishes Guide on Sterility Testing


Effective Removal of DMSO from Stored Samples


ATL Webcast: A Practical Guide to Selecting the Right LIMS


Genetic study reveals how gut bacteria work to keep us healthy


Lonza Establishes Custom Cell Biology Services to Offer Unique Manufacturing and Testing Capabilities


Thermo Fisher Scientific Opens Food Safety Customer Solution Center in India






Can't find what you are looking for?

Search by company or by product

Company Name:




Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd