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BioVision Introduces Three New Assay Kits: Yeast Mitochondria Isolation Kit, Mitochondrial Protein IP Kit & Cytochrome Oxidase Activity Colorimetric Assay Kit
BioVision is thrilled to introduce Three Brand New Assay Kits: Yeast Mitochondria Isolation Kit- A highly efficient kit to isolate intact yeast mitochondria, Mitochondrial Protein IP Kit - For IP/Co-IP/Pull-down of mt. proteins or complexes & Cytochrome Oxidase Activity Colorimetric Assay Kit- Rapid, HTS Kit to measure Cytochrome Oxidase Activity
Mitochondria are the powerhouse of eukaryotic cells. Located in the cytoplasm, they are the sites of cellular respiration, which ultimately generates fuel for the cell's activities. They generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are also involved in other cell processes such as cell division and growth, as well as cell death. Damage to the mitochondria activates signaling pathways that induce apoptosis. Mitochondria also regulate and mediate transport of the metabolites and ions needed for oxidative phosphorylation and maintenance of membrane potential for ATP synthesis. Mitochondrial dysfunction leads to several disorders like cardiac dysfunction, diabetes, aging and neurological disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes that code for mitochondrial components. Thus, mitochondria have several different functions in the cell. Therefore, isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.
Yeast Mitochondria Isolation Kit
BioVision's Yeast Mitochondria Isolation kit utilizes yeast cell wall lysis and homogenization. It is tested for Pichia pastoris & Saccharomyces cerevisiae, but can be used to isolate mitochondria from other yeast as well.
Key features and Benefits of the kit:
- Enables fast and easy purification of highly pure mitochondria from yeast cells.
- The yield is ~150-200 µg of mitochondria in fermentable media from a culture of OD ~20.
- The yield is ~200-250 µg of mitochondria in non-fermentable media (e.g. Glycerol) from a culture of OD ~20.
- Can be used for Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling, Western blot and ELISA.
- Compatible with all yeast cell cultures.
Mitochondrial Protein IP Kit
BioVision’s ready to use Mitochondrial Protein IP Kit's Buffer is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.
Key features and Benefits of the kit:
- Optimized for compatibility with immunoprecipitation (IP and co-IP) and pull-down using tagged proteins.
- Gentle formulation for maintenance of stable mitochondrial complexes.
- Compatible with SDS PAGE, 2D gel, Blue-Native gel, Mass spectrometry.
- Can be used for functional Assays and enzymatic assays.
- Compatible with mitochondria, mitochondrial extract or cell lysate.
Cytochrome Oxidase Activity Colorimetric Assay Kit
Cytochrome c Oxidase (EC 220.127.116.11) or Complex IV is the fourth complex of the Electron Transport Chain located in the mitochondrial (or bacterial) membrane. It provides energy to the cell by coupling electron transport through the Cytochrome c chain with the process of oxidative phosphorylation. Complex IV contains 13 different subunits encoded by both mitochondrial DNA and nuclear DNA. It receives an electron from each of the four Cytochrome c molecules, and transfers it to one oxygen molecule, converting it into two molecules of water. In this process, it also binds to four proton molecules and translocates them across the membrane to establish electrochemical gradient, which is utilized for the synthesis of ATP. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.
Key Features and Benefits of the Kit
- Fast and high-throughput adaptable.
- Convenient measurement of Cytochrome Oxidase enzymatic activity in 96-well plate format.
- Can be used for mitochondrial respiration studies, assembly of the complexes, mitochondria outer membrane integrity.
- Compatible with purified mitochondria or cell/tissue extracts containing mitochondria