Dionex is proud to announce a new method to desalt oligonucleotide or protein samples for either on-line analysis by high-performance liquid chromatography (HPLC) or liquid chromatography-mass spectrometry (LC/MS). Application Update 174: On-Line High-Throughput Desalting to Prepare Samples for Mass Spectrometry demonstrates a simple, fast, and effective process that was completed within 2 min, including sample desalting and flushing, for an oligonucleotide sample and a bovine serum albumin (BSA) sample using a Dionex UltiMate® 3000 HPLC system and Chromeleon® Chromatography Data System (CDS) software.

MS has become a powerful tool for the analysis of complex biochemical samples. These samples often contain high concentrations of salts that interfere with the ionization process and may result in lower peak intensities, compromised peptide/protein detection, and frequent contamination of the ion source. Therefore, it is essential to remove salts from samples before subjecting them to MS detection. Although off-line solid-phase extraction (SPE) is the most common desalting method, it is time-consuming. This application update describes a simple, fast, and effective on-line method using an Acclaim® PolarAdvantage II Guard column for high-throughput sample desalting before MS detection.

For more details, visit www.dionex.com.

Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and sample preparation systems, consumables, and software for chemical analysis. The company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products.

Acclaim, Chromeleon, and UltiMate are registered trademarks of Dionex Corporation.