Innovation Makes High-Parameter Flow Cytometry More Accessible

Beckman Coulter Life Sciences, a Danaher company and a global leader in laboratory automation and innovation, announces the launch of its new IR820 and IR870 infrared dye antibody conjugates. For the first time, research flow cytometry labs have a low noise solution, including single laser excitation, no dye interferences along with negligible overlap with other fluorochrome emissions, and ultralow autofluorescence background.
 
IR820 and IR870 reagents are the industry’s first directly conjugated antibodies for infrared excitation. The reagents are ideal for lineage marker staining, offering phenomenal brightness to clearly identify respective gating antigens, while leaving detection of non-infrared dyes uncompromised due to near-to-complete absence of spectral overlap. Lineage gating markers now can be added, or if already existing in the antibody panel, moved far away from commonly used spectral ranges where typically the most precious dim signals reside.
 
“As high-parameter flow cytometry becomes more mainstream, antibody panel design gets even more crucial due to tightening complex spectral interference patterns that can reduce data quality,” said Pietro Lopriore, Vice President and General Manager of the Flow Cytometry Business Unit. “Our IR820 and IR870 infrared dye conjugates with their low noise design remove complexities and make the infrared spectral range a premium place for lineage markers, allowing for a more flexible antibody panel design. By enabling greater clarity, we’re able to uniquely empower researchers in an exciting new frontier of human phenotyping experiments.”
 
Current flow cytometry fluorochrome conjugates do not cover the infrared range. Lineage markers used for identification of major subpopulations typically reside in the same spectral ranges as probes for weak co-expressed antigens, enabling characterization of subpopulations and their functional status. By this spectral colocalization, typically bright lineage markers may compromise resolution and sensitive detection of their dimmer co-expressed companions.
 
“We recently tested the CD3 IR820 infrared flow cytometry antibody, and we are thoroughly impressed with the results,” finds Dr. Theo Gerdener, Head of immune phenotyping at ABJ Oncology Pretoria, South Africa. “By using markers in these channels, we can reserve the other channels for other additional markers that are less common, which is crucial for complex multi-parameter assays. Given the clear separation and minimal interference with other channels, we highly recommend this infrared antibody for anyone looking to expand their flow cytometry panels.”
 
This addition to the Beckman Coulter Life Sciences reagent portfolio is ideal for users of IR-equipped CytoFLEX LX and S instruments, including spectral with the mosaic detection module, to extend human phenotyping experiments into an easy-to-navigate spectral range. IR820 and IR870 are non-tandem dyes which can be excited at 808 nm and emit with maximal intensity at 835 and 896 nm, corresponding to the 840/20 nm and 885/40 nm or similar bandpass filters. The Research Use Only reagents comprise CD3, CD4, CD8, CD14, CD20 and CD45 specificities and are manufactured under current good manufacturing practices.

About Beckman Coulter Life Sciences

Time is the most critical facet in the laboratory today:  time to get life-saving therapies to patients faster; reclaiming time by automating tedious manual workflows; and saving time otherwise spent addressing erroneous or complex results.
 
At Beckman Coulter Life Sciences, a Danaher company, we are partners in time. We accelerate answers to critical questions through the power of efficiency, saving hours from some workflows and delivering reliable results in centrifugation, flow cytometry, genomic solutions, particle analysis/counters, and liquid handlers.
 
We develop innovations for scientists by scientists, with many of our 3,300+ global colleagues coming from the laboratory with a deep understanding of today’s challenges and complexities. We’re passionate about translating science in partnership with our customers, and our customizable, accessible and sustainable solutions empower them with intuitive workflow efficiencies.
 
Time and again customers put their trust in us, with 400,000+ of our built-to-last products currently delivering results in labs worldwide – all backed by our dependable service and application experts offering educational insights and tailored support.
 
It’s all part of our time-tested approach since 1935 to bring meaningful innovations at the speed of life—and we’re just beginning. Working together, let’s advance human health for a better tomorrow!

 
About Danaher

Danaher is a leading global life sciences and diagnostics innovator, committed to accelerating the power of science and technology to improve human health. Our businesses partner closely with customers to solve many of the most important health challenges impacting patients around the world. Danaher's advanced science and technology - and proven ability to innovate - help enable faster, more accurate diagnoses and help reduce the time and cost needed to sustainably discover, develop and deliver life-changing therapies. Focused on scientific excellence, innovation and continuous improvement, our approximately 63,000 associates worldwide help ensure that Danaher is improving quality of life for billions of people today, while setting the foundation for a healthier, more sustainable tomorrow.

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