Search News Archives
Follow us on
Conferences | Events

Media Partners

 

New Possibilities for Multiphoton Microscopy from Carl Zeiss

Multiphoton Microscopy

Multiphoton confocal microscopes from Carl Zeiss now permit the simultaneous use of two NLO lasers or one laser with an optical parametric oscillator (OPO). Both components are fully integrated and expand the functionality of the multiphoton systems.

In dual laser systems different laser wavelengths simultaneously excite several fluorescent dyes or proteins. Without time loss, users can image specimens with one wavelength and manipulate them in the multiphoton mode with another. The automatic free beam adjustment gives the system a high degree of stability and reproducibility and ensures exact overlay of the two excitation beams. Such dual laser systems are used, above all, in intravital microscopy, e.g. for examining functional correlations in the brain of a mouse.

An OPO increases the excitation range of multi- photon microscopy to up to 1300 nanometers and therefore covers in particular the absorption peak of red fluorescent proteins such as mCherry, mPlum and tdTomato. This efficient, long-wave excitation enables excellent specimen protection. The potentially very high light intensities of the OPO lasers interact with specific structures in the tissue, leading to a doubling and tripling of the oscillation frequency. This non-linear effect of frequency doubling (SHG) occurs, for example. in striated skeletal muscle and collagen. Frequency tripling is especially visible on regions where structures with inconsistent optical density converge. These include lipid-water boundaries - for example, between membrane and cytoplasm.

For further information click here


If you have not logged into the website then please enter your details below.



 


Popular this Month

 

Win a PIPETBOY from the new Integra summer collection!

 

Great Savings off Selected GC Columns

 

CytoSMART raises 5.2 million Euros for further product innovation and growth

 

High Volume Sample Storage in Space Efficient 96-Well Format

 

Cherwell Publishes Guide on Sterility Testing

 

Effective Removal of DMSO from Stored Samples

 

ATL Webcast: A Practical Guide to Selecting the Right LIMS

 

Genetic study reveals how gut bacteria work to keep us healthy

 

Lonza Establishes Custom Cell Biology Services to Offer Unique Manufacturing and Testing Capabilities

 

Thermo Fisher Scientific Opens Food Safety Customer Solution Center in India

 

 


 

 


 

Can't find what you are looking for?

Search by company or by product


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd