Search News Archives
Follow us on
Conferences | Events

Media Partners

 

IDT launches Double-Quenched ProbesTM to increase qPCR sensitivity and precision

Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has introduced a new double-quenched probe, which increases the accuracy and reliability of 5' nuclease qPCR experiments. While traditional probes have 20-30 bases between the dye and quencher, this novel proprietary probe design positions an internal ZEN quencher only 9 bases from the 5' fluorophore. This shortened distance, particularly when combined with the standard 3' quencher, significantly decreases background fluorescence and increases sensitivity. The chemical structure of the ZEN quencher stabilizes duplex formation which allows for its use in previously validated sequences. The improved functionality significantly increases qPCR accuracy and sensitivity when compared to traditional probes. 

Due to the incorporation of ZEN at a fixed position 9 bases from the 5' end, the quencher is always within close proximity of the probe. As such, the initial background fluorescence signal is much lower. This makes subsequent changes easily detectable and functionally increases assay sensitivity. In addition, while traditional probes do not remain well quenched over 30 base pairs, the double-quenched probes maintain a consistently low background even at 40 base pairs or longer. Thus, double-quenched probes result in lower Cq values when compared to traditional probes. This leads to an increase in specificity without any loss in sensitivity or quenching efficiency. This is particularly useful for targets that are AT-rich and require longer probes. 

IDT offers a complete range of standard and custom qPCR products including a free online RealTime PCR design tool which optimizes primer and probe sequence for specific user requirements.

For further information, please visit www.idtdna.com.


If you have not logged into the website then please enter your details below.



 


Popular this Month

 

Win a PIPETBOY from the new Integra summer collection!

 

Great Savings off Selected GC Columns

 

CytoSMART raises 5.2 million Euros for further product innovation and growth

 

High Volume Sample Storage in Space Efficient 96-Well Format

 

Cherwell Publishes Guide on Sterility Testing

 

Effective Removal of DMSO from Stored Samples

 

ATL Webcast: A Practical Guide to Selecting the Right LIMS

 

Genetic study reveals how gut bacteria work to keep us healthy

 

Lonza Establishes Custom Cell Biology Services to Offer Unique Manufacturing and Testing Capabilities

 

Thermo Fisher Scientific Opens Food Safety Customer Solution Center in India

 

 


 

 


 

Can't find what you are looking for?

Search by company or by product


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd