Follow us...

 

Search News Archives

News Channels

 

New Laboratory Products

Lab News

What's going on at Lab Innovations

Research & Case Studies

Microscopy | Image Analysis

Separation Science

Brochures & Literature

Videos

Events | Webinars

 

 

Conferences | Events

Eliminate time-consuming, expensive titrations and reduce variability in your Next Generation Sequencing with a high performance qPCR solution

KAPA.jpgThe new KAPA Library Quantification Kits available from Anachem utilise the power of Quantitative PCR (qPCR) to provide you with an accurate and sensitive method for quantifying Next Generation Sequencing (NGS) libraries.

Standard methods for quantifying NGS libraries have a variety of disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples and are not suitable for high-throughput workflows.

qPCR, however, is a highly sensitive approach which uses a minimal amount of material and eliminates the need for time-consuming and expensive titrations. It specifically quantifies only PCR-competent DNA molecules and due to its high sensitivity, allows for the accurate quantification of low concentration libraries and significantly reduces cluster density variations. It can also be automated for high-throughput applications.  

"Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs".

- The Broad Institute, Cambridge, MA U.S.A.

The KAPA Library Quantification kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include defined, reliable DNA concentration standards and state-of-the-art qPCR reagents, containing a DNA polymerase engineered for SYBR® Green-based qPCR through a process of molecular evolution to provide a rapid, sensitive, and reliable method for quantifying PCR-amplifiable molecules in next-generation sequencing DNA libraries.

Try it for yourself & realise the benefits. Call us now on 01582 747500 or email bioscience@anachem.co.uk for your FREE Sample. But hurry limited samples available.


If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners