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Carl Zeiss Obtains License from University of California for Illumination Technique

zeissCarl Zeiss has received a license from the University of California in San Francisco (UCSF) for the commercialization of "Multidirectional Selective Plane Illumination Microscopy" (mSPIM), an advanced illumination technique for light sheet fluorescence microscopy.

Light sheet fluorescence microscopy is a relatively new application for the life sciences.  It is ideally suited for live imaging of fluorescently labeled specimens up to millimeters in size.  Specimens can be imaged for days under certain physiological conditions and with minimum photo-induced damage.

The mSPIM technique was developed by Dr. Jan Huisken at UCSF.  It reduces absorption and scattering artifacts and provides an evenly illuminated focal plane.  By alternating illumination of the sample from multiple sides, mSPIM overcomes two common problems in light sheet imaging techniques: shadowing effects in the excitation path and spreading of the light sheet by scattering in the sample.

The agreement grants Carl Zeiss the right to integrate the mSPIM technology in its microscopy systems.  The first commercial light sheet fluorescence microscope (LSFM, also known as "selective plane illumination microscope" or "SPIM") for multidimensional, ultrafast and long-term timelapse imaging of live specimens is currently being developed at Carl Zeiss in Germany.

Together with Carl Zeiss' innovative concepts for light sheet microscopy, the licensing of mSPIM represents another big step forward in 3D microscopy of living specimens. This benefits diverse fields such as developmental biology, cell biology, neurobiology, stem cell research and marine biology.

For more information visit www.zeiss.com


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