Follow us...

 

Search News Archives

Channels

News

 

 

View Channel

Laboratory Products

 

 

View Channel

Special Offers and Promotions

 

Microscopy | Image Analysis

 

 

View Channel

Separation Science

 

 

View Channel

Coronavirus (COVID-19)

 

 

View Channel

Research & Case Studies

 

 

View Channel

Conferences | Events

Cutting-edge Cryo-EM reveals key insight into vital DNA repair process

publication date: Apr 1, 2021
 | 
author/source: University of Glasgow

cuttingedge-cryoem-reveals-key-insight-into-vital-dna

New research, using cutting-edge cryo-electron microscopy (CryoEM), has revealed key insights into a vital DNA repair process, which is implicated in resistance to cancer treatments.

Led by the University of Glasgow and published in Nature Structural Biology, the research is based on data and models collected from the Scottish Centre for Macromolecular Imaging (SCMI) and was conducted with colleagues at the University of Dundee.

The study looks at a toxic type of DNA damage called inter-strand crosslinks, which is normally repaired through a process initiated by a single molecule of ubiquitin – a protein commonly found in humans, animals and plants – being attached to each of the affected strands of DNA. In order to complete the DNA repair process the ubiquitin molecule must also be successfully removed from the damaged site – a process known as deubiquitination.

Now, for the first time, researchers are able to show at a molecular level, the exact snapshot in time when the ubiquitin molecule is about to be removed by the targeting enzyme USP1 (Ubiquitin carboxyl-terminal hydrolase). To do this scientists used the cutting-edge electron microscope at the SCMI, and with the data are now able to understand how this complex process occurs.

Understanding how USP1 interacts with ubiquitin during the removal process is considered to be scientifically important and opens the door to further research.in the area that could have impacts on cancer and other diseases. In cancer cells, efficient functioning of USP1 can help repair any damage caused by drug therapies, thereby making treatment of the disease less successful. As a result, USP1 has beenidentified as a potential drug target for overcoming cancer resistance to treatment.

Professor Helen Walden, lead author of the study and professor of structural biology at the University of Glasgow, said: “The developments in cryo-EM over recent years have revolutionised structural biology, and we are really excited to capture this important complex, and how this will allow us to understand the DNA repair on a deep molecular level.”

The new £5m SCMI is hosted by the University of Glasgow and is part of the Medical Research Council-University of Glasgow Centre for Virus Research (CVR) and is the result of collaboration between researchers from Glasgow, Edinburgh, Dundee and St Andrews.

The SCMI is a structural biology centre, home to a cutting-edge electron microscope – the first of its kind in Scotland – which will be used to image biological molecules at the atomic level.

The technology is being used to support vital research into diseases posing the greatest threat to human and animal health, providing greater capabilities in areas such as vaccine development, cancer research, and drug design and discovery.

The study, ‘Structural basis of FANCD2 deubiquitination by USP1-UAF1’ is published in Nature Structural Biology. The work is funded by the European Research Council and the Medical Research Council (MRC).

 

Learn more about the SCMI


 



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.