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Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer reinvigorates high-end protein research with expanded analysis capabilities

Scientists in academic, government and biopharmaceutical laboratories who are faced with increasingly complex research challenges now have access to a digitally advanced Thermo Scientific Orbitrap Tribrid mass spectrometry (MS) platform that includes a new high-performance MS with inherent flexibility and built-in intelligence to enable critical insights and breakthroughs.

The new Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer features important advancements that improve system sensitivity over previous generations and expand its ability to characterize and quantify complex biomolecules and biological systems. These enhancements are ideal not only for native omics and translational research, but also for the structural and biopharmaceutical analysis of intact proteins and their complexes.

The real-time capabilities of the Orbitrap Eclipse Tribrid mass spectrometer use the latest, built-in intelligence-driven data acquisition strategies to enhance experimental efficiency and ultimately accelerate tandem mass tagging (TMT)for multiplexed quantitative analysis of proteomes. Peptide spectra are searched in real-time against a study-specific database. Those that match then proceed for further analysis, significantly increasing the speed of analysis and accuracy of results. In addition, the new system extends structural analysis up tom/z8,000, which enables the isolation and selective dissociation of protein complexes into their individual components, as well as revealing further insights about their exact structure.

Thermo Fisher Scientific showcased its new Orbitrap Eclipse Tribrid instrument during the 67th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics, being held June 2–6, in the International Ballroom at the Omni CNN Center Hotel, Atlanta, Georgia.

Steven Gygi, PhD, professor of cell biology at Harvard Medical School, said, "My research group is investigating subtle proteome-wide responses to environmental, pharmacological, pathological or genetic changes, which can often be challenging and time-consuming to detect and quantify. With the real-time searching capabilities, the Orbitrap Eclipse Tribrid mass spectrometer helps us to quickly decide the mass spectra we need to acquire during our experiments, which drastically reduces the number of scans we need to perform and the time it takes to obtain the results that matter most to us."

"With protein-based discoveries advancing at a remarkable pace, leading researchers understandably demand that analytical technologies keep up with their progress and advances in experimental design," said Iain Mylchreest, vice president, R&D, for Thermo Fisher’s analytical instruments business. "The new Orbitrap Eclipse instrument fundamentally improves the sensitivity, speed and accuracy of quantitative proteome analysis, enabling scientists to comprehensively define proteoforms and protein complexes at an unprecedented level of detail."

Users of the Orbitrap Eclipse Tribrid mass spectrometer can benefit from:

• Improved sensitivity and speed over previous generations, with real-time search functionality providing rapid and accurate TMT analysis.
• Ability to perform intact/top-down experiments and simplify spectra with Proton Transfer Charge Reduction to extract important structural insights.
• Powerful higher-order mass spectrometry (MSn) analysis of protein complexes and their component parts, thanks to novel high mass range functionality that expands instrument mass range tom/z8,000.
• New Thermo Scientific SureQuant internal standard-triggered methods for ultra-sensitive targeted protein assays provides the ability to rapidly develop and deploy targeted assays for large protein panels. SureQuant methods are available for the Thermo ScientificSureQuant AKT/mTOR Pathway Kit and the Biognosys PQ500TM kit for the targeted quantitation of 500 plasma proteins.
• Significantly expanded protein coverage through advances in instrument design and use of theThermo Scientific FAIMS Pro interface to enhance precursor ion selectivity. Management of this interface is fully incorporated into control software for easy integration.
• Unified hardware and instrument control software with the newThermo Scientific Orbitrap Exploris 480 mass spectrometer and Thermo Scientific Triple Quadrupole platform of mass spectrometers

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