Providing a simple and robust solution for many laboratories, protein
sequencing utilizing automated Edman degradation chemistry remains a vital
method of producing unambiguous data on biopharmaceutical or biochemical
materials. An Edman sequencer, such as Shimadzu's innovative
PPSQ series
instrument, is ideal for producing N-Terminal Sequencing information for
proteins and peptides and is less complex to use than many alternative mass
spectrometry-based techniques.
Shimadzu's PPSQ series achieves greater simplicity and reliability in
the automatic determination of amino acid sequences by employing
state-of-the-art HPLC analysis coupled with high sensitivity detection and
powerful new acquisition/analysis software. N-Terminal sequencing is a key
part of the ICH Q6B guidelines for characterization and confirmation of
biopharmaceuticals for new marketing applications. The PPSQ Series has
been specifically developed to simplify the generation of customized reports
for information relating to estimated sequences and yield rates which can
easily be displayed in graphical form to enable users to select amino acids for
their calculations.
Ideal for supporting QA applications in biopharmaceutical and
biochemistry companies, the Shimadzu PPSQ series is also well suited for
university departments looking to run protein sequencing studies.
"Shimadzu is the only company producing a fully supported Edman
Sequencing solution now that Applied Biosystems has discontinued its Procise
Edman Sequencer globally," explained Stuart Phillips, Shimadzu UK's LC/LCMS
Business Manager. "If any UK
company still requires an Edman sequencer service then Shimadzu UK's
PPSQ Series is the only proven solution in production capable of performing
Edman N-Terminal sequencing."
"A further benefit is that the PPSQ Series offers cost savings of up to
50 percent compared with the Procise system. Add to that the PPSQ's
simplified operation enables better reproducibility coupled with better
baseline stability and you simply have a winning combination."
By using Shimadzu's highly acclaimed HPLC, the PPSQ series protein
sequencers are able to separate PTH-amino acids isocratically which improves
baseline stability to allow high-sensitivity analysis of PTH-amino acids.
Isocratic sequence analysis provides more stable retention times.
Analysis in isocratic mode allows eluents to be recycled, which reduces the
amount of mobile phase required and reduces waste disposal rates and in turn
leads to significant cost savings.
The PPSQ series' software features control functions for the reaction
unit and HPLC, enabling easier sequence analysis of proteins and peptides.
Easy-to-use data analysis functions simplify the reprocessing of chromatograms
as well as the overlaying of multiple chromatograms which all helps in the
automatic estimation of amino acid sequences.
For more information visit
www.shimadzu.co.uk
About Shimadzu
Shimadzu is a world leader in the
design and manufacture of products and services within a broad range of fields
including bioresearch. The company's commitment to the philosophy of
"contributing to Society through Science and Technology" forms its common
managing concept throughout the world: "Solutions for Science since
1875". Instruments for analysis and measurement in industry and research,
environmental testing and other bio-related equipment, and semi-conductor and
media related equipment and software services are within Shimadzu's portfolio.
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