The crosslinking process utilizes short wave (254 nm) UV light to covalently bond nucleic acids to either nitrocellulose or nylon membranes.

Spectronics Corporation manufactures advanced, versatile and accurate crosslinkers to avoid the loss of nucleic acids during the hybridization process, a consistent, irritating problem for lab technicians. The Select™ XLE-Series is a high quality, inexpensive ultraviolet crosslinker that addresses this issue. It covalently binds nucleic acids to membranes in under 30 seconds — 240 times faster than vacuum-oven baking.

The unit is able to identify and analyze trace amounts of DNA/RNA with far greater sensitivity, accuracy and speed than with conventional analysis. Lab technicians will also appreciate the exceptional accuracy and precision irradiation, even compensating for aging UV tubes when output diminishes over time.

Researchers rely on this unit for eliminating PCR contamination, nicking ethidium-bromide-stained DNA in agarose gels, gene mapping for creating cleavage-inhibiting thymine dimers, RecA mutation screening in E.coli, UV sterilization and miscellaneous UV-dosage applications.

The Select Series crosslinker features a “smart” user-friendly, fully programmable microprocessor, built-in “help” messages and an auto-repeat function that make its operation amazingly fast and simple. The fully enclosed chamber protects users from UV exposure. It features five 8-watt tubes and is available in a choice of 254nm, 312nm and 365nm versions.

Headquartered in Westbury, New York, Spectronics Corporation invented fluorescent leak detection in 1955, and remains the world’s leading manufacturer of ultraviolet equipment and fluorescent dyes. Spectroline^® cutting-edge, top-quality, industry-awarded lamps, dyes, radiometers and diagnostic tools are built to exacting standards, and are used for literally dozens of markets, some of which include Industrial, Air Conditioning & Refrigeration, Laboratory, Semiconductor, Nondestructive Testing and Forensics. Spectronics Corporation is dedicated to upholding high standards in design manufacturing, ensuring customer satisfaction and developing innovative technologies that make the world a better place.

more about spectronics

more news from spectronics