Combining PMT-based Microplate Detection with Automated Digital Widefield Fluorescence Microscopy

Cytation 3 Imaging Reader

Multi-mode microplate readers are an indispensable laboratory instrument that enable many applications to be performed on multiple samples.

Life science applications involving cells are increasingly desired due to higher biological relevance associated with functional readouts. Microplate readers can provide quantification of a specific target, such as a protein or its post-translational modification, or phenotypes such as cell death through apoptosis or necrosis. Quantification is provided relative to assay controls and/or standards using dispersive optical detection and light collection with photomultiplier tubes (PMT). Conversely, imaging offers greater contextual information regarding the cell population in the microplate well. Using fluorescent probes, multivariate data can be acquired that can provide both qualitative and quantitative data and spatial information using similar dispersive optical elements in addition to microscope objectives to magnify and CCD cameras to capture images. Visualization of the cells in the well provides qualitative confirmation of probe activity and whether that activity is occurring in the right place in the cell; image analysis routines such as multi-color cell segmentation allows for quantitative cell sub-population analysis, such as the percent of cells in the field of view expressing the phenotype of interest. 

The Cytation™3 Cell Imaging Multi-Mode Reader provides all of these capabilities in one small footprint unit. In addition, the PMT-based optics can be used to rapidly screen cells and using fluorescence intensity threshold criteria, decide whether specific wells should be imaged.

The following application notes demonstrate the full capabilities of Cytation3 providing a new look at cell-based assays.

Application Notes:

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