Thermo Fisher Scientific has developed an ion chromatography method with suppressed conductivity detection for sensitive determination of inorganic anions and carboxylic acids (fluoride, acetate, formate, mesylate, chloride, nitrate, succinate, malonate, sulfate, and oxalate) in airborne particulate matter (PM2.5).
Application Note 1107: Determination of Anions and Carboxylic Acids in Urban Fine Particles (PM2.5) demonstrates that this new method can determine more analytes relevant to air contamination than either of the two standard methods currently in use. The determination is performed using a high-pressure ion chromatography system, the Thermo Scientific™ Dionex™ ICS-5000+ Reagent-Free™ HPIC™ system, equipped with a...
In collaboration with scientists from the European Union Reference Laboratory for Dioxins and PCBs in Feed and Food, Thermo Fisher Scientific has developed a simple, reliable and robust method for detecting and confirming low levels of dioxins in food and feed samples using a triple quadrupole gas chromatograph-mass spectrometer (GC-MS/MS) system equipped with an autosampler.
Application Note 10406: Validation of GC-MS/MS for Detection and Confirmation of Low-Level Dioxins illustrates the excellent selectivity and sensitivity of this GC-MS/MS System for low levels of dioxins, even in complex matrices. Dioxins are a group of chemically-related compounds, including polychlorinated dibenzodioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and some dioxin-like polychlorinated biphenyls (PCBs). These compounds are...
Thermo Fisher Scientific has developed two robust methods to identify uronic acids in acid-hydrolyzed wood samples, with each method using a different specialized anion-exchange column as demonstrated in Application Note 1091: Determination of Uronic Acids and Wood Sugars in Wood-Based Hydrolysates.
Method 1 is a single high-performance anion-exchange chromatography with pulsed amperometric detection method that uses a mono- and disaccharide column, the Thermo Scientific™ Dionex™ CarboPac™ PA20 column, for wood sugars and uronic acids. Method 2 is a faster method that uses an oligosaccharide column...
In collaboration with scientists from the European Union Reference Laboratory for Dioxins and PCBs in Feed and Food, Thermo Fisher Scientific has developed a simple, reliable and robust method for detecting and confirming low levels of dioxins in food and feed samples using a triple quadrupole gas chromatograph-mass spectrometer (GC-MS/MS) system equipped with an autosampler.
Application Note 10406: Validation of GC-MS/MS for Detection and Confirmation of Low-Level Dioxins illustrates the excellent selectivity and sensitivity of this GC-MS/MS System for low levels of dioxins, even in complex matrices. Dioxins are a group of chemically-related compounds, including polychlorinated dibenzodioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and some dioxin-like polychlorinated biphenyls (PCBs). These compounds are...
Thermo Fisher Scientific has developed an efficient and simple high-performance liquid chromatography method for the determination of dicyandiamide in milk powder using an ion-exclusion chromatography column that is capable of rapidly analyzing polar compounds such as aliphatic organic acids and alcohols in complex and high-ionic-strength samples.
Thermo Fisher Scientific has developed a pH-gradient-based method that provides excellent resolution for monoclonal antibody (MAb) charge variants.
Application Note 1092: Separation of Intact Monoclonal Antibody Sialylation Isoforms by pH Gradient Ion-Exchange Chromatography demonstrates that this approach is more convenient and straightforward than the isoelectric focusing method routinely used for protein sialylation profiling. This new method uses a cation-exchange protein column on a biocompatible analytical liquid chromatography system; a separate project is...
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography (HPLC) method using a mixed-mode column, the Thermo Scientific™Acclaim™ Mixed-Mode WAX-1 column, with ultraviolet detection for a rapid and sensitive determination of acesulfame in vinegar.
Application Note 1104: Rapid and Sensitive Determination of Acesulfame in Vinegar demonstrates that this approach offers the advantages of simple sample preparation, good method reproducibility, and a wide linear calibration range. This new method also achieves a detection limit much lower than that specified by the Standardization Administration of China. Acesulfame is extensively used as a sugar substitute in foods and...
The Clinical and Laboratory Standards Institute (CLSI) has released a new document, Liquid Chromatography-Mass Spectrometry Methods; Approved Guideline (C62-A).
This document provides guidance to the clinical laboratorian for the reduction of interlaboratory variance and the evaluation of interferences, assay performance, and other pertinent characteristics of clinical assays. C62-A emphasizes particular areas related to assay development and presents a standardized approach for method verification that is specific to mass spectrometry (MS) technology. This document provides guidance for the development and verification of liquid...
Thermo Fisher Scientific has developed a method to analyze food and environmental matrices for the presence of alkylphenol compounds using a GC-MS/MS system with automated selected reaction monitoring (AutoSRM).
Application Note 10407: Analysis of Alkylphenols Using GC-MS/MS and Automated SRM Development demonstrates the combined analytical power of the Thermo Scientific TRACE 1310 GC, the Thermo Scientific TSQ Duo triple quadrupole mass spectrometer and the Thermo Scientific Dionex Chromeleon Chromatography Data System (CDS) software to detect and quantify alkylphenol compounds in water and solid samples....
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography (HPLC) method with fluorescence detection for sensitive and rapid determination of benzo[a]pyrene in meat products.
This method uses on-line solid-phase extraction (SPE) for sample preparation instead of the commonly used off-line SPE method. Application Note 1093: Determination of Benzo(a)pyrene in Sausage and Preserved Ham demonstrates that this approach reduces costs associated with the SPE cartridge, labor, time, and reagents; and results are more consistent because the cleanup step is performed by automated on-line SPE rather than...
Thermo Fisher Scientific has developed an ion chromatography (IC)-based method to simultaneously determine lithium, sodium, and calcium in lithium carbonate without handling hazardous reagents.
Application Note 1090: IC Assay for Lithium, Sodium, and Calcium in Lithium Carbonatedemonstrates accurate determinations of these three analytes by separation on a cation-exchange column and detection by suppressed conductivity. This approach offers a fast (25 minutes), simple, accurate, and robust alternative to the time-consuming assays for these three analytes in the current United States Pharmacopeia (USP) monograph...
Thermo Fisher Scientific has developed a method that uses solid-phase extraction (SPE) well plates to improve sample preconcentration prior to analysis while maintaining high levels of precision, accuracy, recovery, and sample cleanliness.
Application Note 20941: SOLAμ for Pre-Analysis Sample Concentration demonstrates the ability to decrease the lower limit of quantitation by a factor of 20 without the need for lengthy evaporation procedures that may compromise analytical results. By performing a preconcentration step during sample preparation, analysts can reduce workflow, provide stability for analytes susceptible to loss or degradation during evaporation/reconstitution, and...
Thermo Fisher Scientific has developed an efficient high-performance liquid chromatography method that uses fluorescence detection to achieve a rapid and sensitive determination of rhodamine B in cosmetics.
Application Note 1102: Rapid and Sensitive Determination of Rhodamine B in Cosmetics demonstrates the advantages of this method using a rapid separation liquid chromatography (RSLC) column on an RSLC system. This approach provides good method reproducibility (retention time reproducibility relative standard deviation [RSD] of 0.09% and peak area reproducibility RSD of 0.75%) and a wide linear calibration range (from 2 to 1000 μg/L)...
Thermo Fisher Scientific has developed a precise, automated gas chromatography (GC) method for static headspace analysis of residual solvents in flexible packaging, using a headspace autosampler capable of multiple headspace extraction (MHE).
Application Note 10396: Static Headspace Analysis of Residual Solvents in Flexible Packaging and Quantitation with Multiple Headspace Extraction Following EN 13628-1: 2002 demonstrates the combined analytical power of the Thermo Scientific TRACE 1310 GC and the Thermo Scientific TriPlus 300 Headspace Autosampler to detect and quantify solvents that may leach from adhesives in flexible plastic packaging into the food products the packaging was...
Thermo Fisher Scientific has developed an efficient ion chromatography (IC) method with on-line sample cleanup for the rapid and sensitive determination of p-toluenesulfonic acid in water-insoluble drugs.
The on-line cleanup step provides the benefits of efficient matrix elimination, time savings, and convenience. Application Brief 165:Determination of p-Toluenesulfonic Acid in Water-Insoluble Drugs shows that this approach—performed on an integrated IC system that includes sample preparation capability and uses an ion chromatography column designed for separation with sodium carbonate/sodium biocarbonate eluents—demonstrates good linearity with a...
Thermo Fisher Scientific has developed a high-resolution hydrophobic interaction chromatography (HIC) method that can separate a fusion protein from a truncated version and a structural isomer.
Application Brief 171: High-Resolution Separation of a Fusion Protein demonstrates that two different HIC columns—the Thermo Scientific™ ProPac™ HIC-10 column and the new MAbPac™ HIC-10 column—can each perform this challenging separation. While the ProPac HIC-10 column is an established column for the analysis of monoclonal antibody post-translational modifications, the MAbPac™ HIC-10 column exhibits the best performance....
Thermo Fisher Scientific has developed two rapid and robust high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) methods for the accurate determination of common sugars in acid-hydrolyzed wood samples.
Application Note (AN) 1089: Determination of Carbohydrates in Acid Hydrolysates of Wood demonstrates that this approach achieves high-resolution separation of even the sugar pair rhamnose-galactose that has been challenging to resolve using existing HPAE-PAD methods. Both methods use a new polymeric column, the Thermo Scientific™ Dionex™ CarboPac™ SA10-4μm column, configured with a new system, the...
Thermo Fisher Scientific has updated an ion chromatography (IC) method to more easily determine total choline (free and bound forms) in 17 samples representing all forms of infant formula, adult nutritionals, and pediatric formulas (powder, ready-to-feed products, and liquid concentrates).
Application Update (AU) 193: Choline in Infant Formula and Adult Nutritionals, a Single Laboratory Validation demonstrates that this simple yet robust approach decreases sample preparation time while accurately determining choline in these products. The method detailed in AU 193 runs on an IC system that requires only a source of degassed DI water for generation of high-purity eluent, thus simplifying operation while increasing precision and...
Thermo Fisher Scientific has developed a simple, efficient, sensitive, and reproducible capillary ion chromatography (IC) method for determining thiosulfate and pyrophosphate in crayfish wash powder.
Application Note 1088: Determination of Thiosulfate and Pyrophosphate in Crayfish Wash Powder demonstrates that this application is easily executed using a Reagent-Free™ IC system capable of high-pressure IC controlled by data management software. The analyst simply adds water and samples to separate thiosulfate and pyrophosphate on a specialized anion-exchange column, the Dionex IonPac AS19 capillary column, thus...
Thermo Fisher Scientific has developed a simple, rapid, reliable, and accurate high-performance liquid chromatography (HPLC) method for the simultaneous determination of amlodipine and besylate in an amlodipine besylate drug substance.
Application Note 1087: Simultaneous Determination of Amlodipine and Its Counterion Besylate by HPLC demonstrates that this method requires only 8 minutes per analysis using a column designed for pharmaceutical analysis and ultraviolet detection. In addition, combining the analysis of the active pharmaceutical ingredient (API) and counterion into a single method saves times, reduces mobile phase consumption, and minimizes waste. Amlodipine besylate is a...
Thermo Fisher Scientific has developed a high-performance anion-exchange chromatography with pulsed amperometric detection method, based on AOAC Official Method 2011.18, to determine free and bound myo-inositol in infant formula and adult nutritionals.
Application Note (AN) 1083: Determination of Myo-Inositol (Free and Bound as Phosphatidylinositol) in Infant Formula and Adult Nutritionalsdemonstrates a column-switching technique used to remove strongly retained carbohydrates in the sample, thereby reducing run time. Pulsed amperometric detection with a gold on polytetrafluoroethylene disposable electrode offers high sensitivity while eliminating the need for sample derivatization and electrode polishing...
Thermo Fisher Scientific has demonstrated that a hydrophobic interaction chromatography (HIC) column, the Thermo Scientific™ ProPac™ HIC-10 column, is suitable for separating low-concentration species of an intentionally oxidized MAb from the native MAb.
The same method can be used to separate the papain fragments from the intentionally oxidized MAb, as well as the papain fragments of the native MAb. Application Note 1084: Separation of Papain Digests of Oxidized Monoclonal Antibodiesdetails how the ProPac HIC-10 column can be used to resolve antigen-binding fragments (Fab) and nonantigen-binding fragments (Fc) generated by papain digestion, as well as oxidized Fab/Fc from native Fab/Fc...