Monthly Newsletter

Industry-Leading Adapters Mitigate Index Hopping and Spread of Signal in Next Gen Sequencing Applications

PerkinElmer, Inc., a global leader committed to innovating for a healthier world, has announced its NEXTflex® unique dual index barcodes, the latest addition to the NEXTflex family of library prep solutions.* The NEXTflex unique dual index barcodes are 192 barcoded adapters that provide unmatched data security in sequencing applications for use on Illumina® platforms.

This advanced system is designed to specifically mitigate index hopping and spread of signal, a known phenomenon associated with multiplexing that impacts next-gen sequencing technologies. Instruments utilizing a patterned flow cell and exclusion amplification technology experience increased levels of sample mis-assignment as a result.  These adapters address the important issue of sample misidentification, thereby enabling assurance of data integrity.

By allowing researchers to pool multiple libraries in a single flow cell lane, multiplexing with the NEXTflex barcoded adapters also significantly increases processing capacity, while reducing costs.

“The NEXTflex unique dual index barcodes are designed to increase sensitivity and flexibility, thereby allowing users to accurately differentiate between samples and maximize the use of a Next Generation Sequencing run.” said Arvind Kothandaraman, Director of the NGS Product Portfolio, Applied Genomics, PerkinElmer.  “We take pride in enabling researchers make scientific breakthroughs by providing innovative tools for their Next Generation Sequencing workflow.”

*PerkinElmer’s NEXTflex unique dual index barcodes are for research use only and not for use in diagnostic procedures. For more information, please click here.    

PerkinElmer offers solutions spanning the genomics workflow. These include nucleic acid extraction, liquid handling, sample preparation, DNA/RNA quantitation, library preparation, data analysis, and applications expertise.