Porvair Filtration Group has
launched a new web microsite (
www.chromatrap.com)
dedicated to providing detailed information on its ground breaking solid state
Chromatin Immunoprecipitation (ChIP) technology for the Epigenetics
market. The web microsite provides an informative background as to what
ChIP technology is about and introduces the new solid state approach pioneered
by researchers at Porvair Filtration Group.
Porvair's new Chromatrap® Pro-A technology depends on a porous inert polymer
called BioVyon
TM. Each Chromatrap® spin column contains a BioVyon
TM disc with the
internal pore structure covalently bonded with Protein A (BioVyon
TM Protein A).
Complex mixtures containing the chromatin fragments tagged with the antibody of
choice are flowed through the column assisted by centrifugation. The
column retains the chromatin of interest whilst unwanted debris is flushed
away. Purified chromatin is eluted ready for downstream processing and
analysis.
A collaborative case study on the site compares the Chromatrap protocol with
results from using a Protein G Magnetic Beads technique. Under optimal
conditions, the Chromatrap protocol resulted in a 25.5 fold increase in the
percentage DNA pull-down using the exact same sample of MCF7 breast cancer
cells, targeting the RNA polymerase II protein bound to the GAPDH target gene.
A further independent case study compares Porvair Chromatrap technology with
alternative traditional ChIP methods (Sepharose and Dynabeads). In this
study Chromatrap columns were shown to provide a greater level of enrichment of
the DNA of interest due to low levels of background binding compared to
specific antibody binding. Also the DNA pull down (percentage input) was
significantly higher for the Chromatrap columns than for the other matrices in
these assays.
For further information or to discuss evaluating the new Chromatrap
TM ChIP assay
technique please contact Porvair Filtration Group on +44-1489-864330 /
Kevin.Quinlan@chromatrap.com
Porvair Filtration Group is an international leader in the development and
supply of materials and products for applications in filtration and separation.
Their expertise in a wide range of media and the dedication of their design,
manufacturing, sales, test and research teams ensure they are at the forefront
of filtration technology, delivering world class performance to the Aerospace,
Defence, Nuclear, Energy, Chemical Process, Industrial Process, Water
Treatment, Printing and Life Science markets. With offices and manufacturing
sites located in the UK, Europe and the USA combined with a world-wide network
of market representatives and distributors, they pride themselves on their
continuous innovation and research to meet global demands.
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