A new, self-contained GENE
CLONING SYSTEM which enables real-time fractionation and recovery of
nucleic acids in real time has been introduced by electrophoresis specialists CLEAVER
SCIENTIFIC (CSL). The RunVIEW system is claimed to be the most
innovative and versatile tool for gene cloning for real-time horizontal gel
electrophoresis.
RunVIEW combines the flexibility
and high resolution capability of the MSCHOICE system with the time and
space-saving convenience of having the power supply and gel illuminator
integrated within one highly compact, bench top unit. The system includes
everything except the chemicals and reagents to run horizontal SYBR and EtBr
stained gels in real-time.
Using runVIEW is simple. First,
cast a gel featuring two rows of wells with one matching pair of the
preparatory combs supplied, before transferring the blue light transparent gel
tray to the MSCHOICE tank located on the base unit. Then, add sufficient buffer
just to cover the gel and remove the combs to load the DNA samples into the
upper row of wells ‘loading tier'. Replace the lid to connect the MSCHOICE tank
to the integrated power supply before applying the voltage and switching on the
blue LED gel illuminator. Electrophoresis can be viewed through the
bluVIEW lid's viewing pane as the samples migrate in real-time to the second
row of wells, ‘extraction tier'. Once the DNA bands of interest enter the
extraction tier, simply stop the power supply, remove the lid and harvest the
DNA by pipette.
RunVIEW's blue light enables
scientists to track DNA without the detrimental effects of UV transillumination,
including mutagenesis which can compromise cloning efficiency, while the
system's capacity to provide real-time visualisation of electrophoresis means
DNA can be tracked as it migrates through the gel. This allows the user to
judge precisely when the band of interest is ready for extraction. Furthermore,
running DNA into a well containing buffer effectively purifies the DNA of
agarose, eliminating the need for time-consuming gel excision and purification
techniques that can also result in sample loss. Once harvested, the DNA need
only be cleaned by a straightforward ethanol precipitation when it's ready for
further digestion or ligation.
RunVIEW removes the need for
expensive pre-cast commercial gels as it works with standard EtBr, SYBR green
and SYBR safe gels cast within the 15 x 7, 15 x 10 or 15 x 15cm MSCHOICE gel
trays. Finally, as a self-contained system, with the base unit housing the
power supply and blue LED gel illuminator, this compact, dual-voltage and
portable device allows electrophoresis, gel visualisation and extraction to be
performed at the bench, without the inconvenience of having to transport gels
into a darkroom elsewhere in the laboratory.
For more information
click here
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