Providing fast and accurate gene assembly
The latest issue of
Integrated DNA
Technologies' (IDT's) DECODED
newsletter highlights how New England Biolabs (NEB) researchers
were able to expedite the cloning of a 2.1 kb gene into a linearized pET21-a
bacterial expression vector using 5 gBlocks
TM
Gene Fragments that spanned the entire 2.1 kb
sequence. Cloning, sequencing, and characterization of protein activity took
less than 4 days, shorter than necessary for a third party to synthesize the
complete gene and provide a finished construct.
Peichung Hsieh, Application
Scientist at New England Biolabs, Inc. commented "The use of gBlocks Gene
Fragments was simple and cost-effective, saved time, and resulted in fewer
errors than the assembly of DNA from single-stranded synthetic
oligonucleotides. These benefits ensure that Gibson Assembly cloning using
gBlocks Gene Fragments will be a method we will continue to work with in the
future".
gBlocks
Gene Fragments are designed to reduce the time, error rate, and costs associated with
producing single gene constructs or multiple genes within metabolic pathways.
Used in conjunction with the recently introduced NEB Gibson Assembly
TM Master
Mix, NEB reduced both the hands-on time and the total turnaround time of some
of their cloning workflows.
You can read more about
NEB's
protocol in the latest issue of the IDT quarterly
newsletter,
DECODED. For more information about the complete IDT product
portfolio, and to sign up to receive DECODED, visit
www.idtdna.com.
About IDT
Integrated DNA Technologies (IDT) is the largest
supplier of custom nucleic acids in the United States, serving academic,
government, and commercial researchers in biotechnology, clinical diagnostics,
and pharmaceutical development. IDT's primary business is the manufacture of
custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and
ships an average of 36,000 custom oligos per day to more than 86,000 customers
worldwide. IDT manufacturing locations include facilities in Coralville, Iowa;
San Diego, Calif.; and Leuven, Belgium.
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