- Uses novel membrane
technology for rapid and simplified sample preparation
- Infrared-based spectrometry
system provides accurate, sequence-independent analysis that requires minimal
sample size
- Overcomes limitations of
traditional protein quantitation methods such as Lowry and Bradford assays
Merck Millipore,
the Life Science division of
Merck
KGaA of Darmstadt, Germany, today launched the Direct Detect
TM system for
rapid, simplified protein quantitation. The Direct Detect
TM system
exploits Merck Millipore's membrane expertise to enable infrared-based
measurement of amide bonds in protein chains, an intrinsic component of every
protein, without relying on amino acid composition, dye-binding properties, or
redox potential. Conventional assays based on UV-Vis spectroscopy rely on
absorbance by a protein's aromatic amino acids and therefore have limited
utility.
"In addition to its analytical powers, the Direct Detect
TM
system provides a groundbreaking departure from traditional sample prep requirements
typical of biomolecular quantitation. After samples are spotted on assay cards,
they can be stored in ambient conditions without appreciable change in
readout," describes John Sweeney, Head of Life Science Business Field, Merck
Millipore. "Another important advantage over conventional assays is that
the Direct Detect
TM calibration standard curve requires generation once, which
provides additional time savings and ease of use."
The system employs a hydrophilic polytetrafluoroethylene
(PTFE) membrane designed to be transparent in most of the infrared spectral
region and enables application of biomolecule solutions directly onto the
membrane. The Direct Detect
TM system delivers more universally applicable
and faster protein quantitation, requiring minimal sample consumption. System
accuracy and precision are comparable with results obtained by amino acid
analysis, an accurate standard for quantitation, yet can be time-consuming and
often costly.
Because the system relies on IR-based detection of
biomolecules, users can obtain accurate and reproducible protein quantitation
in presence of reducing agents and detergents. As a result, the Direct
Detect
TM system can measure protein concentrations from 0.2 mg/mL to 5 mg/mL
within seconds, without any bio- or immunochemical staining, directly from
samples, including buffered solutions.
The Direct Detect
TM system can also be used to provide
information on non-protein sample components, such as lipids and nucleic acids.
For more information, please visit
http://www.millipore.com/directdetect
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