- High-throughput, automation compatible, 96-well
plate-based method for chromatin immunoprecipitation
- Streamlined protocol and proprietary buffer system
provides excellent sensitivity and lower backgrounds compared to
conventional approaches
- Reliable results using as few as 10,000 cells per well,
from cultured cells or tissue
Merck Millipore, the Life Science division of Merck, has introduced the Magna ChIP
TM HT96 kit for
high-throughput chromatin immunoprecipitation (ChIP). Using the kit, ChIP
can be performed on up to 96 samples in a single experiment using as few as
10,000 cells per well. The optimized protocol uses a single,
proprietary buffer for sonication, immunoprecipitation and washing which yields
excellent sensitivity and lower backgrounds than conventional ChIP methods.
The Magna ChIP
TM HT96 protocol
has been designed and optimized for efficient immunoprecipitation and recovery
of DNA. Consequently, in many cases, a separate DNA purification step is not
required for downstream analysis.
"ChIP is used for mapping
the
in vivo
distribution of proteins associated with chromosomal DNA. For many laboratories,
ChIP can be technically demanding, requiring robust antibodies, high quality
reagents, and careful planning and handling." notes John Sweeney, Head of
Life Science Business Field, Merck Millipore. "The Magna ChIP
TM HT96 kit
was designed to address these challenges when processing a larger number of
samples. The protocol and reagents simplify the standard ChIP procedure,
improve the reproducibility and robustness of the ChIP process, and minimize
the "hands-on" steps in ChIP."
The Magna ChIP
TM HT96 kits
utilize a magnetic protein A/G bead blend that provides multiple advantages
over single-bead approaches. In contrast to kits with either protein A or
protein G, the Magna ChIP
TM A/G beads allow affinity capture of a broader range
of antibody isotypes. In addition, the magnetic properties of these beads
permit rapid processing of ChIP samples to maintain high fold enrichment when
used with multichannel pipettes and magnetic separation stand or with the use
of an automated liquid handler.
The kit is available with or
without control antibodies and qPCR analysis primers.
Merck Millipore offers a wide
selection of highly specific antibodies demonstrated to work in ChIP
(ChIP-qualified antibodies) as well as a collection of rigorously
ChIP-validated antibodies and control primer sets known as ChIPAb+
TM kits.
For more information on Magna
ChIP
TM HT96, ChIP-validated antibodies and additional technologies, please visit
www.millipore.com/epigenetics.
About Merck Millipore
Merck Millipore is the Life
Science division of Merck KGaA of Germany and offers a broad range of
innovative, performance products, services and business relationships that
enable our customers' success in research, development and production of
biotech and pharmaceutical drug therapies. Through dedicated collaboration on
new scientific and engineering insights, and as one of the top three R&D
investors in the Life Science Tools industry, Merck Millipore serves as a
strategic partner to customers and helps advance the promise of life science.
Headquartered in Billerica,
Massachusetts, the division has around 10,000 employees, operations in 67
countries and 2011 revenues of EUR 2.4 billion. Merck Millipore operates as EMD
Millipore in the U.S. and Canada.
Note: Merck KGaA or
Merck shall mean Merck KGaA, Darmstadt, Germany