Stable phosphoramidites for use in triplex, antisense and therapeutic
applications
Link Technologies
Ltd, an ISO 9001:2008 certified specialist oligonucleotide reagent
manufacturer, has launched two new modified phosphoramidite ranges to
complement its existing extensive portfolio of products to support
oligonucleotide synthesis. For triplex, antisense and gene targeting studies,
the new RNA-type phosphoramidites provide nuclease resistance and stability,
while the new ethyl
phosphoramidites improve oligonucleotide stability and delivery into cells,
making them ideal for therapeutic applications.
Building on from the existing
range of standard nucleobase RNA phosphoramidites and CPG supports, the
modified RNA-type phosphoramidites comprise two new 2'-OMe RNA products,
2'-OMe-I and
2'-OMe-T.
Oligonucleotides containing these modifications are ideal for triplex,
antisense and gene targeting studies, as they form more stable hybrids with
complementary RNA strands compared to equivalent DNA or RNA strands.
Furthermore, when used in gene targeting studies, the addition of 2'-OMe
residues into triplex forming oligonucleotides (TFO's), confers the same
nuclease resistance and stability as seen with duplexes.
Oligonucleotides synthesised from
Link's
new ethyl phosphoramidites (available with standard nucleobase protection)
have a neutral charge and slightly lipophilic character, which improves their
delivery into the cell. Perfect for use in therapeutic applications,
oligonucleotides containing these ethyl groups are nuclease resistant and have
been shown to inhibit protein expression and cell growth when taken up by
liposomes.
Dr Catherine McKeen, Technical
Manager at Link Technologies, commented: "We understand the importance of
evolving our product offering to reflect the developing needs of our customers.
As part of this, the new modified phosphoramidites
provide increased nuclease resistance and stability, extending the experimental
capabilities available to our customers."
For more information
please visit the Link Technologies website at
www.linktech.co.uk
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