The CESI
8000 High-Performance Separation-ESI Module with OptiMS Technology from Beckman
Coulter, Inc. consists of the first commercial CESI sprayer combined with new
capillary electrophoresis instrumentation specifically designed for mass
spectrometry (MS). It gives researchers a robust, high-sensitivity interface of
capillary electrophoresis (CE) with MS, while providing a broad range of
analyte coverage complementary to LC-MS and GC-MS.
OptiMS
Technology integrates an intrinsically low flow CE separation (as low as 10
nL/min.) with electrospray ionization (ESI) in a single, dynamic process that
takes place within a capillary. The new
process is referred to as CESI, reflecting the combination. To achieve CESI,
the distal end of the capillary is porous to ion flow, allowing the
electrophoresis circuit to be bridged and ESI to be initiated within the same
device.
CESI-MS
is capable of generating separations of peptides with a high peak capacity
(>320), allowing the analysis of very complex samples at the proteome
level. The ultra-low flow rates used by
CE effectively reduce ion suppression and make it possible to detect
post-translational modifications (PTMs) like phosphorylation and sialylation,
which typically hamper efficient ionization.
High-separation efficiency
and sensitivity permit the detection of peptides present in low abundance at
concentrations in the picoMolar range.
The selectivity of CE in front-end separation further enables the characterization of intact proteins, their isoforms,
cleaved fragments and complexes.
CESI 8000
with OptiMS Technology from Beckman Coulter also provides fast and efficient
separations of drugs, drug metabolites, organic acids, amino acids, low
molecular weight amines, peptides, nucleic acids and nucleosides. Molecules
often missed or altogether undetectable by technologies like reversed phase
LC-MS, now become visible with CESI 8000.
"The CESI 8000 was
developed in collaboration with mass spectrometry researchers who work in a
variety of applications and who sought to expand the range of analytes detected
while improving overall assay sensitivity," noted Jeff Chapman, director of Beckman Coulter Discovery
Products. "The result is a front-end
separation system that generates data complementary to HPLC and provides
scientists with important, previously unavailable information."
Easy switching
between LC-ESI-MS and CESI-MS allows the addition of CESI to existing
workflows. The CESI 8000 is installed on
a height-adjustable, gear-driven portable lab bench so it can be rapidly set to
the proper height of a mass spectrometer.
Plug-and-spray adapters are available for top brand nanosources.
For more information, visit
www.beckmancoulter.com.
Beckman Coulter, Inc., based in California, USA,
develops, manufactures and markets products that simplify, automate and
innovate complex biomedical tests. More than 275,000 Beckman Coulter systems
operate in laboratories around the world, supplying critical information for
improving patient health and reducing the cost of care. Recurring revenue,
consisting of consumable supplies (including reagent test kits), service and
operating-type lease payments, represents about 80 percent of the company's
2010 revenue of $3.7 billion.
In development. For Laboratory Use
Only; not for use in diagnostic procedures.
Beckman Coulter and the stylised logo are trademarks of Beckman
Coulter, Inc. and are registered in the USPTO.
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