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Field Flow Fractionation of Proteins, Macromolecules and Nanoparticles

publication date: Apr 27, 2016
 | 
author/source: Postnova Analytics GmbH

The Postnova AF2000 MultiFlow is a high performance Flow Field-Flow Fractionation (FFF) platform for separation of proteins, macromolecules and nanoparticles.

postnovaModular in design, the AF2000 MultiFlow incorporates the combined experience, expertise and technological advances from Postnova Analytics' nearly two decades of leadership in FFF. Incorporating a range of FFF modules in a single integrated system to provide universal separation, the AF2000 MultiFlow offers more flexibility, better performance and more robust results than any system before.

The AF2000 MultiFlow platform is based on the Flow FFF principles using a unique crossflow field design in which samples are separated by their dynamic diffusion on the basis of molar mass or particle size. Because of this design and the absence of any stationary phase, field-flow fractionation on the AF2000 MultiFlow can be performed without exerting shear forces and stress on the molecules or particles being separated.
The AF2000 can be run with different eluents, wide ranging temperature conditions and the same system can be used for running planar Asymmetric Flow FFF (AF4) or circular Hollow Fiber Flow FFF separations (HF5). Consequently the AF2000 MultiFlow is an ideal platform for the characterization of proteins, antibodies, aggregates, vaccines, VLPs, liposomes, nanoparticles and natural or synthetic polymers.

The AF2000 MultiFlow is available in a series of different models. Various add-on modules, extra options and special FFF detectors are available, such as Multi-Angle Light Scattering (MALS) and Dynamic Light Scattering (DLS). All Postnova FFF modules and detectors have been especially developed for FFF and are perfectly optimised not only for the use with the AF2000 MultiFlow, but also for the other Postnova FFF systems. Additionally the AF2000 Series has been designed for easy interfacing with high-end detection technologies, such as ICP-MS, from other suppliers that may already be at use in your laboratory.

Founded in 1997, Postnova Analytics is the inventor and leading international supplier of Field-Flow Fractionation (FFF) systems for markets including biopharmaceuticals, polymers, materials, nanotechnology and environmental sciences. Leveraging its unique modular FFF - Light Scattering Platform, Postnova has been able to provide high performance solutions to a wide range of applications. Today the company's growing product portfolio includes Flow FFF, Centrifugal FFF, Thermal FFF, Size Exclusion Chromatography (SEC), MALS and DLS Light Scattering.  Postnova is located and headquartered in Landsberg am Lech (Munich, Germany) and has subsidiaries in the USA and UK. These offices in conjunction with a highly qualified and trained distribution network provide informed local support to customers worldwide.


more about postnova analytics





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