Dolomite, a world leader in the design and manufacture of microfluidic devices, has successfully developed an innovative Droplet System for the encapsulation of single cells or beads into droplets which benefits a wide range of applications including single cell analysis, high throughput screening and droplet PCR.
The system represents a major breakthrough in microfluidics
by providing users with the tools required to perform assays on individual cells in picolitre
volume environments. This novel technology was demonstrated by feeding 2.8µm
diameter magnetic beads into monodisperse 100µm diameter droplets at a ratio of
1 bead per droplet with minimal bead losses.
Featuring
Dolomite's standard microfluidic components, the new system allows users to
load single cells or beads into droplets. The Mitos P-Pump allows stirring of
the input fluid reservoir, to provide an even distribution of beads and
delivers a pulseless and stable flow for excellent control of droplet volumes. Reliable
and leak-free connections are made from pump to tube
to chip using Dolomite's Multiflux® Linear Connectors.
Droplets
are
isolated in an inert fluorocarbon carrier stream to prevent
cross-contamination
and are stabilized with Pico-Surf
TM, a biocompatible surfactant designed
specifically for generating and stabilizing pico-droplets.
For
further information on Dolomite's Micro Droplet Systems as well as the complete
portfolio of microfluidic products including chips, connectors/ interconnects,
valves, pumps and custom devices please visit
www.dolomite-microfluidics.com.
The Dolomite Centre Limited
Established
in 2005 as the world's first Microfluidic Application Centre, Dolomite focused
on working with customers to turn their concepts for microfluidic applications
into reality. Today, Dolomite is the world leader in solving microfluidic
problems. With offices in the UK and US and distributors throughout the rest of
the world, its clients range from universities developing leading-edge
analytical equipment, to manufacturers of chemical, life science and clinical
diagnostics systems.
Dolomite
is pioneering the use of microfluidic devices for small-scale fluid control and
analysis, enabling manufacturers to develop more compact, cost-effective and
powerful instruments. By combining specialist glass, quartz and ceramic
technologies with knowledge of high performance microfluidics, Dolomite is able
to provide solutions for a broad range of application areas including
environmental monitoring, clinical diagnostics, food and beverage, nuclear,
agriculture, petrochemical, cosmetics, pharmaceuticals and chemicals.
Furthermore Dolomite's in-house micro-fabrication facilities that include clean
rooms and precision glass processing facilities allow to prototype and test all
solutions rapidly which ensures a faster development cycle and reduces the time
to market.
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