Dionex Demonstrates Simplified Sample Preparation Method for HPAE-PAD Analysis

Dionex announces a new method that simplifies sample pretreatment for small-scale peptide N-glycosidase F (PNGase F) digests that use protein denaturation. Application Update 176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis demonstrates that this new pretreatment method makes samples amenable to high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) oligosaccharide analysis with the CarboPac® PA200 column, thereby making it possible to monitor the oligosaccharide contentº of a glycoprotein. This approach overcomes the limitations of using traditional size-exclusion columns with low- to medium-molecular weight cut-off resins for sample pretreatment. Most size-exclusion methods are time consuming, require large amounts of sample, and run the risk of losing charged sugars.

The biotechnology industry has already produced a number of glycoprotein therapeutics and more are being investigated for their therapeutic potential. The companies that manufacture these products must understand and monitor the oligosaccharide content of the glycoprotein to ensure that it is consistent from one lot of therapeutic to another. HPAE-PAD is one of the tools used to study and monitor glycoprotein oligosaccharides.

Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and sample preparation systems, consumables, and software for chemical analysis. The company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products.

For further information please visit www.dionex.com

CarboPac is a registered trademark of Dionex Corporation.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.