Thermo Fisher Scientific
Inc. is pleased to announce an economical new method that uses high-performance
liquid chromatography (
HPLC) with
charged aerosol and
UV detections to determine steviol glycosides
in consumer sweeteners. Application Note 293:
Steviol Glycoside Determination by HPLC with Charged
Aerosol and UV Detections Using the Acclaim Trinity P1 Column demonstrates that only 3-9 mL of mobile
phase/sample are required, compared to 25-60 mL/sample for the Joint FAO/WHO
Commission on Food Additives (
JECFA) and U.S. Pharmacopeia (
USP) methods, respectively. The reduction in
mobile phase use saves both time and resources during mobile phase preparation
and also reduces waste.
Using the Thermo Scientific Acclaim Trinity P1 column, the proposed method
resolves the steviol glycosides of interest. Quantification of the two
principal glycosides, stevioside and rebaudioside A, can be accomplished by
both detection methods. Charged aerosol detection has the advantage of
distinguishing additional components in the sample that are not UV absorbing,
allowing for a flexible method to check sweetener purity.
This application note and many others can be found at
www.thermoscientific.com/dionex under the
Documents tab.
About Thermo Fisher
Scientific
Thermo Fisher Scientific
Inc. is the world leader in serving science. Our mission is to enable our customers
to make the world healthier, cleaner and safer. With revenues of nearly $11
billion, we have approximately 37,000 employees and serve customers within
pharmaceutical and biotech companies, hospitals and clinical diagnostic labs,
universities, research institutions and government agencies, as well as in
environmental and process control industries. We create value for our key
stakeholders through two premier brands, Thermo Scientific and Fisher
Scientific, which offer a unique combination of continuous technology
development and the most convenient purchasing options. Our products and
services help accelerate the pace of scientific discovery, and solve analytical
challenges ranging from complex research to routine testing to field
applications.
All trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!
Request your free copies HERE
Popular this Month
Top 10 most popular articles this month
Today's Picks
Looking for a Supplier?
Search by company or by product
Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.
Lab Bulletin is published by newleaf marketing communications ltd.