In situ ultramicrotome turns ZEISS FE-SEM into high speed 3D cell
and tissue imaging system
Today, the collaboration
between Carl Zeiss Microscopy and Gatan, Inc. was officially announced. Carl
Zeiss Microscopy is a leading supplier of light and electron microscopes.
Gatan, Inc. is the world's leading manufacturer of instrumentation and software
for the enhancement of electron microscopes' operation and performance. In this
joint project, the companies will promote the development and sales of a system
which provides high-resolution 3D data of resin embedded cell and tissue
samples.
Member of the Board and
General Manager BioSciences Division of Carl Zeiss Microscopy Dr. Bernhard
Ohnesorge is very pleased about the collaboration: "The 3View versions of
our FE-SEMs MERLIN and SIGMA VP support the general application trend in
biomedical research of 3D imaging and reconstruction to understand context in
tissues. Since the knowhow of Gatan and Carl Zeiss complement each other
ideally these systems are the easiest to use and fastest tools for a biologist
to follow this trend into electron microscopy resolution and dramatically
increase work efficiency."
It enables
the automated serial blockface imaging of embedded samples (e.g. cells or
tissue ) with slice thickness down to 15 nanometers. Thus, image stacks
containing hundreds, or even thousands of sequential slices may be generated,
and a three-dimensional profile of the sample with nanometer resolution built
up. Joel Mancuso, Applications Manager at Gatan, Inc. recognizes the benefits:
"The 3View system simplifies three-dimensional electron microscopy providing
researchers with limited electron microscopy expertise an opportunity to
collect high resolution volumetric datasets by removing the difficult step of
cutting and collecting ultrathin sections." The first pilot customers are
already using the systems successfully, among them Renovo Neural Inc.
(Cleveland), offering 3DEM commercial services.
For further information
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