publication date: Jul 10, 2012
|
author/source: BERTHOLD TECHNOLOGIES
Berthold Technologies, specializing in bioanalytical
instruments for the research and the diagnostics markets, presents the 2nd
generation of its multi-label TriStar²
microplate reader.
TriStar² employs the new patent pending
optical concept ALL-4-ONE, for the first time enabling luminescence,
fluorescence and absorbance measurements with highest respective sensitivity as
known from dedicated instruments. TriStar²
is also capable of
reading FRET, BRET and BRET².
In comparison to its predecessor the optics
has been further improved and an improvement in detection limits in
fluorescence has been achieved
- Less
than 6 amol ATP per well
- Less
than 0.3 fmol Fluorescein per well
The instrument is characterized by an
universal detector with extremely low noise for fluorescence and luminescence
measurements and it is equipped with a photo diode for absorbance readings in
addition. Furthermore optical filters can be used for luminescence measurements
enabling BRET (e.g. functional assays for GPCR research) and multi-coloured
luciferase reporter gene applications.
TriStar² can be fitted with up to 3 reagent
injector - based on the proven and most accurate
JET technology - and
temperature control for the microplate compartment. Two of the injectors have
their tips in reading position enabling the measurement of extremely fast
reactions. New to the instrument is the reagent compartment placed at the front
where the reagents are easily accessible and where they can be kept cooled by
the addition of crushed ice when needed. For increased sample throughput
TriStar² can integrated into lab automation systems. Special reagent mounts
provide a safe fixation of small reagent vials und ensure complete consumption
of the reagents through inclination of the vial.
Thus, TriStar² microplate reader offers a
multitude of possibilities and a wide range of applications. Measurements of
enzyme activities, phagocytosis, Calcium flux, cell viability, apoptosis,
immuno assays, protein and DNA concentrations and protein-protein interactions
are only a few of the many uses of TriStar².
The unit is operated via the proven and
intuitive ICE software. Single and multiple endpoint readings are possible as
well as kinetic and scanning measurements. The data are displayed in numerical
and graphical formats and may be exported into EXCEL or printed.
For more information about the TriStar²
microplate reader,
click here
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