Porvair
Filtration Group working in collaboration with the Department of Biological
Sciences, University of Essex, UK has produced an alternative, high performance
Solid Phase Affinity Matrix for Chromatin Immunoprecipitation (ChIP) assays.
The research at Essex University evaluating the new BioVyon - Protein A
material in ChIP assays is published in the May 2011 issue of Analytical
Biochemistry.
Over the past 8 years - researchers at the University of Essex and Porvair
Filtration Group have pioneered techniques to chemically functionalise the
surface of microporous High Density Polyethylene (HDPE) to enable its use in a
growing number of new biochemical applications. Chemical functionalisation of
Porvair Filtration's unique HDPE material (BioVyon
TM) can endow it with internal
surface properties than can be individually configured to capture and separate
target species out of difficult mixtures. This has opened up many possibilities
in the field of Biosciences where molecules of interest such as DNA, RNA,
proteins etc can be selectively pulled out of complex mixtures of biological
origin. Chromatin immunoprecipitation (ChIP) is an important technique in the
study of DNA/protein interactions. The ChIP procedure, however, has limitations
in that it is lengthy, can be inconsistent, and is prone to non-specific
binding of DNA and proteins to the bead-based solid-phase matrices that are
often used for the immunoprecipitation step.
Researchers at the University of Essex and Porvair Filtration have examined the
utility of a new matrix for ChIP assays, BioVyon Protein A, a solid support
based on porous polyethylene. In ChIP experiments carried out using two
antibodies and seven DNA loci, the performance of BioVyon Protein A was
significantly better, with a greater percentage of DNA pull-down in all of the
assays tested compared with bead-based matrices, Protein A Sepharose, and
Dynabeads Protein A. Furthermore, the rigid porous disc format within a column
made the BioVyon matrix much easier to use with fewer steps and less equipment requirements,
resulting in a significant reduction in the time taken to process the ChIP
samples. In summary, BioVyon Protein A provides a column-based assay method for
ChIP and other immunoprecipitation-based procedures; the rigid porous structure
of BioVyon enables a fast and robust protocol with higher ChIP enrichment
ratios.
Porvair Filtration has recently launched the first in a range of kits for ChIP
assays based on the functionalised BioVyon
TM - Protein A called Chromatrap.
For further information please visit
www.chromatrap.com
or contact Porvair Filtration Group on +44-1489-864330 /
kevin.quinlan@chromatrap.com.
Porvair Filtration Group is an international leader in the development and
supply of materials and products for filtration and separation applications.
Their expertise in a wide range of media and the dedication of their design,
manufacturing, sales, test and research teams ensure they are at the forefront
of filtration technology, delivering world class performance to the Life
Science, Aerospace, Defence, Nuclear, Energy, Chemical Process, Industrial
Process, Water Treatment and Printing markets. With offices and
manufacturing sites located in the Europe and the USA combined with a
world-wide network of market representatives and distributors, they pride
themselves on their continuous innovation and research to meet global demands.
Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!
Request your free copies HERE
Popular this Month
Top 10 most popular articles this month
Today's Picks
Looking for a Supplier?
Search by company or by product
Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.
Lab Bulletin is published by newleaf marketing communications ltd.