Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

New Column Designed for Increased Sensitivity for Rapid LC/MS Analyses

publication date: Jun 24, 2015
 | 
author/source: Thermo Fisher Scientific

Unique capillary design optimizes sensitivity and minimizes carryover for high performance protein identification

Thermo A new column for liquid chromatography-mass spectrometry (LC/MS) analysis uses a unique capillary design to offer labs with limited samples and complex mixtures a fast and high-resolution solution for the identification of protein samples, facilitating high sensitivity proteomics and biotech applications.

The Thermo Scientific ProSwift C4 RP-5H column incorporates a exceptionally designed, low-pressure C4 monolith with a porous structure, enabling faster low-flow separations without sacrificing resolution compared with typical porous bead based media. Offering a wide range of operational flow rates, alternative selectivity and low carryover, the ProSwift C4 RP-5H is suited for rapid LC/MS analysis of protein samples.

The new column’s porous copolymer gives the flow channels higher permeability in the stationary phase than particulate columns, resulting in increased separation efficiency. Due to this structure, smaller i.d. columns of 50 μm can be used for analyses, increasing sensitivity.

“The new ProSwift C4 RP-5H column is a great addition to our capillary portfolio as it solves the issues of carryover that can be experienced when analyzing hydrophobic protein samples” said David Sarracino, manager of biomarker workflows at Thermo Fisher’s Biomarkers Research Initiatives in Mass Spectrometry (BRIMS) Center. “The ProSwift C4 RP-5H column is available in a range of column formats for nano, capillary and micro flow chromatography, offering excellent resolution for large, hydrophobic intact proteins across biopharma and research proteomics. This enables flow optimization for increased MS sensitivity as well as rapid separation of monoclonal antibody and complex protein samples.”

The ProSwift C4 RP-5H column will be exhibited for the first time at the 42nd International HPLC Conference in Geneva, Switzerland, Booth 14, June 21–25.

The new monolith uses a butyl (C4) methacrylate co-polymer that is less hydrophobic than PS-DVB columns, meaning there is less column fouling. This is important for MS users where sensitivity is higher compared to UV and carryover can cause interference.


More about the ProSwift C4 RP-5H columns


About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science, with revenues of $17 billion and approximately 50,000 employees in 50 countries. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive support. For more information, please visit www.thermofisher.com.


 


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events